Article Text

Download PDFPDF
Other
Detection of Treponema pedis associated with digital dermatitis in Mediterranean buffalo (Bubalus bubalis)
  1. Jacopo Guccione1,
  2. Giovanni Della Valle1,
  3. Christian Carcasole2,
  4. Peter Kuhnert3 and
  5. Maher Alsaaod4
  1. 1 Department of Veterinary Medicine and Animal Productions, University of Napoli Federico II, Napoli, Italy
  2. 2 Veterinary Practitioner, Latina, Italy
  3. 3 Vetsuisse Faculty, Institute of Veterinary Bacteriology, University of Bern, Bern, Switzerland
  4. 4 Farm Animal Clinic, Vet Suisse, Bern, Switzerland
  1. Correspondence to Dr Giovanni Della Valle; giovanni.dellavalle{at}unina.it

Abstract

Clinical outcomes compatible with digital dermatitis (DD) were observed in a second lactation, milking Mediterranean buffalo (MB). The animal was sharing the free stall with other 99 milking MBs separated by a fence from a pen harbouring 40 milking crossbreed dairy cows. The macroscopic lesion observed on the right rear limb was categorised as M4.1 stage and associated with a severe lameness (locomotion score 5). In the same farm, DD was also observed in 14/40 cows. Nested PCR analysis of genomic DNA isolated from the centre lesion’s biopsy showed the presence of Treponema pedis. Sequence analysis of 494 bp of the PCR fragment revealed 100 per cent sequence identity to the T pedis-type strain, confirming the PCR result’s specificity and for the first time the presence of the bacterium in MB. In order to establish the spread of the DD, a farm inspection was performed to identify risk factors and preventive strategies.

  • foot conditions
  • buffalo
  • digital dermatitis
  • lameness
View Full Text

Statistics from Altmetric.com

Footnotes

  • Contributors All authors have contributed substantially to the care of the animal described in this case, writing of the report, and reviewing and editing of the final report. All authors have approved the final manuscript.

  • Funding The authors have not declared a specific grant for this research from any funding agency in the public, commercial or not-for-profit sectors.

  • Competing interests None declared.

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Data sharing statement No additional data are available.

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.