Postarrival quarantine testing of a consignment of imported miniature donkeys (Equus asinus) with a duplex Taylorella equigenitalis/asinigenitalis real-time quantitative PCR (qPCR) assay and bacteriological culture identified T asinigenitalis in one donkey stallion from the third set of samples collected at weekly intervals. Following transportation to an approved quarantine station, further testing showed the second companion donkey stallion to be additionally positive for T asinigenitalis. Daily topical treatment included the off-label application of a bovine intramammary antimicrobial preparation to the predilection sites with concurrent in-treatment sampling for qPCR to determine treatment end point. Treatment successfully eliminated colonisation with the organism in both donkey stallions within seven days. This report strongly supported multiple sampling opportunities and the inclusion of a duplex qPCR during postimportation screening. The modified treatment regimen appeared to facilitate the rapid interval to elimination of the organism and the prompt determination of treatment end point.
- Taylorella asinigenitalis
- miniature donkeys
- topical intramammary antimicrobial
- South Africa
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Contributors All authors contributed equally to this manuscript.
Funding The authors have not declared a specific grant for this research from any funding agency in the public, commercial or not-for-profit sectors.
Competing interests None declared.
Provenance and peer review Not commissioned; externally peer reviewed.
Data availability statement Data are available upon request.
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